Candida albicans structural and secreted proteins modulate CD4/CD8 ratio in tumor infiltrating lymphocytes of spontaneous adenocarcinoma bearing mice

Candida albicans is one of the most important opportunistic pathogens that suppress immunologic mechanisms of the host. It is speculated that structural and secretory proteins of C. albicans have immunomodulatory effects in cancer. To evaluate the effects of C. albicans structural and secreted proteins on intratumoral CD4/CD8 ratio as well as the survival rate in BALB/c tumor model. Structural and secretory proteins from C. albicans were isolated and examined for their effects on tumor growth and survival of adenocarcinoma bearing mice. The results indicated that in mice treated with C. albicans structural protein, the survival rate significantly decreased compared with the control groups. Also, mice treated with secretory proteins showed a decrease in survival rate but it was not statistically significant [p>0.05]. Investigating the frequency of tumor infiltrated CD4+ and CD8+ T lymphocytes indicated that the percentages of tumor infiltrated CD4+ T lymphocytes in response to structural and secreted proteins were higher compared to the control groups. Our study suggests that C. albicans structural and secreted proteins modulate intratumor T lymphocyte infiltration

Peripheral giant cell granuloma: a review of 123 cases

Background: peripheral giant cell granuloma is one of the reactive hyperplastic lesions of the oral cavity, which originates from the periosteum or periodontal membrane following local irritation or chronic trauma. The purpose of this study was to present the clinical characteristics of peripheral giant cell granuloma in a group of Iranian population

Methods: a series of 123 consecutive confirmed cases of peripheral giant cell granuloma after biopsy were evaluated. Age, sex, anatomic location, consistency, etiologic factor, pain and bleeding history, color, surface texture, and pedicle situation were recorded and were analyzed by chi-square test and values were considered to be significant if P < 0.05

Results: age ranged from 6 to 75 years [mean 33 years]. Women affected more than men [M/F 1:1.1]. Peripheral giant cell granuloma was seen in the mandible more than in the maxilla and in the anterior region more than in the posterior region. In most cases, lesions were pink, pedunculated and had non-ulcerated surface. In less than half of the cases, there was no history of bleeding and also pain was rarely reported. Calculus was the most common etiologic factor

Conclusion: the results confirmed that the clinical features of peripheral giant cell granuloma in a group of Iranian population are almost similar to those reported by other investigators

[Evaluation of cell proliferation and interleukine-4 and interferon gamma level measurement in Balb/c with experimental fibrosarcoma tumor by combination of Gp96-tumor peptide and naloxan]

In spite of the increasing progress in tumor treatment by current methods like surgery, chemotherapy and etc, medical sciences are unable to treat tumors. In this respect, immunology has opened a new window for tumor treatment; nowadays tumor immunotherapy is an accepted strategy for treatment of some tumors at least in some animal models. The goal of this study is the evaluation of immunotherapy using gp96- tumor peptide complex and its combination with naloxon as an opioid receptor antagonist to achieve of cellular immunity against tumors. In this study firstly, gp96 - tumor peptide complexes were purified from WEHI164 cells line using srivastava method. In the next stage, the mice, made tumoric before by the injection of tumor cells, then were divided in to four groups. Control group were injected by PBS, test group1 were injected by naloxon, test group2 were injected by gp96 - tumor peptide complex and test group3 were injected by combination of naloxon and gp96 - tumor peptide complex. To evaluation the efficacy of vaccination, after several days, tumor volume was recorded; then the mice were killed and the spleanic cells were extracted in sterile condition. MTT test was done for cells proliferation study. Supernatant of cultured cells were collected and assayed by ELISA kits for measuring IL-4 and IFN- gamma. Result of protein purification had showen, purified gp96 Isoform has Molecular Weight of 66 kilo dalton.Results of tumor volume had shown that, there is no significant difference between test and control groups. Results of MTT test had shown that, there is no significant difference between test and control groups. IL-4 assay study had showed that, there is no significant difference between test group1, group2 and control group but test group3 has significantly decreased in IL-4 amount when compared with control group. Results of IFN-gamma assay showed that, there is no significant difference between test group1 and control group, but test group2 and group3 has significantly increased in IFN- gamma amount when compared with control group. It can be concluded from this study is that, prophylactic immunotherapy of tumor by combination of gp96-tumor peptide complex and naloxon, can increase IFN- gamma, and, probably in a higher dosage, it may stimulate immune system more to become more potent to even decrease tumor volume

Comparative study of zataria multiflora and anthemis nobelis extracts with myrthus communis preparation in the treatment of recurrent aphthous stomatitis

Recurrent aphthous stomatitis [RAS] is a prevalent and complicated disorder and its management is directed toward treatment of symptoms. The purpose of this study was to compare the efficacy of three herbal preparations in the management of RAS. One hundred and one out of 115 patients with minor aphthae were selected and randomly divided into four groups. Groups A, B and C received topical preparations of Zataria multiflora, Anthemis nobilis and a 50% [v/v] mixture of Zataria multiflora and Anthemis nobilis respectively. Groups D [positive control] received Myrthus communis [Myrtle, mouth rinse], which reported to be efficient in the management of RAS. The time of pain elimination and the duration of the thorough healing were recorded. Mean time of pain elimination showed significant differences [p<0.01] between groups A [3.00 +/- 1.14 day], C [3.08 +/- 1.84 days] and D [4.30 +/- 2.12 days] with group B [5.20 +/- 3.11 days]. The mean duration of healing also showed significant differences [p<0.03] between groups A [6.00 +/- 2.80 days], C [6.70 +/- 2.70 days] and D [7.60 +/- 3.10 days] with B [8.70 +/- 3.90 days]. No significant differences were observed between groups A and C with group D [positive control]. The result obtained for treatment with Z. multiflora [group A] was similar to that of group C, but better than M. communis [group D]. Findings of this study revealed that Z. multiflora extract showed better effects than M. communis which is reported to be effective in the treatment of RAS. It is concluded that the Z. Multiflora extract is an effective product for the management of minor aphthae